The background of the project is the remarkable contribution of our collaborator, Dr. Ferdinand Regner, from the Department of Grapevine Breeding at HBLA u. BA für Wein und Obstbau Klosterneuburg. Dr. Regner has made significant strides in the field by constructing and studying a genetic linkage map of grapevines. This map was developed using the pseudo-testcross strategy, analyzing 92 progeny resulting from a cross between WR and Sirius.
To gain insights into a specific defect, the segregation pattern of this trait was compared with that of 251 molecular markers, including 237 simple sequence repeat (SSR) markers and 14 randomly amplified polymorphic DNA (RAPD) markers. These markers played a vital role in constructing the genetic maps, which were categorized into 20 linkage groups. The maternal (WR) and paternal (Sirius) maps were aligned to create a gene consensus map. This comprehensive map facilitated the identification of a QTL (Quantitative Trait Loci) within linkage group 11. These loci were found to potentially correlate with visual symptoms of magnesium deficiency and the concentration of magnesium in grape zone leaves. Notably, two SSR markers exhibited a strong affinity for the core region of the Mg QTL.
Additionally, Dr. Regner’s research group conducted perennial leaf analyses on 43 WR genotypes, exploring micro- and macroelements. Through their meticulous investigations, they discovered significant differences among these genotypes.
Dr. Regner’s pioneering work and expertise serve as the foundation of our project. His genetic linkage map, the identification of the Mg QTL, and the leaf analyses provide valuable insights into the genetic factors influencing magnesium deficiency, its visual effects, and the magnesium concentration in grape zone leaves. These significant findings propel our project forward as we strive to enhance grapevine health and advance viticulture practices.